pRSET-pr-mEos4b (mEosFP4b-V69T)
(Plasmid #99226)

• Purpose: Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).
• Depositing Lab: Periklis Pantazis
• Publication: Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi: 10.1002/anie.201706121.

Backbone

• Vector backbone: pRSET
• Backbone size w/o insert (bp): 2922
• Total vector size (bp): 3606
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pr-mEos4b
• Alt name: prmEos4b, mEos4b-V69T
• Species: Synthetic
• Insert Size (bp): 684
• Mutation: V69T for primed conversion applications
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: GGCCGATTCATTAATGC
• 3′ sequencing primer: GCTAGTTATTGCTCAGCGG

Resource Information

• Supplemental Documents:
  • pRSETa_ mEos4b-69T.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry