pRSET-pr-mEos3.2 (mEosFP3.2-A69T)
(Plasmid #99224)

• Purpose: Bacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).
• Depositing Lab: Periklis Pantazis
• Publication: Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi: 10.1002/anie.201706121.

Backbone

• Vector backbone: pRSET
• Backbone size w/o insert (bp): 2883
• Total vector size (bp): 3564
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pr-mEos3.2
• Alt name: prmEos3.2, mEos3.2-A69T
• Species: Synthetic
• Insert Size (bp): 681
• Mutation: A69T for primed conversion applications
• Promoter: T7

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GGCCGATTCATTAATGC
• 3′ sequencing primer: GCTAGTTATTGCTCAGCGG

Resource Information

• Supplemental Documents:
  • pRSET-mEos3.2-A69T.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry