pHR-Tre3G-29xGGGGCC-12xMS2
(Plasmid #99149)

• Purpose: Encodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector
• Depositing Lab: Ron Vale
• Publication: Jain et al Nature. 2017 Jun 8;546(7657):243-247. doi: 10.1038/nature22386. Epub 2017 May 31.

Backbone

• Vector backbone: pHR-TRE3G
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 8700
• Total vector size (bp): 9790
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 29xGGGGCC 12xMS2 hairpins
• Species: Synthetic
• Insert Size (bp): 1000
• Promoter: TRE3G

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: AGCTCGTTTAGTGAACCGTCAGATC
• 3′ sequencing primer: CATAGCGTAAAAGGAGCAACA

Resource Information

• Supplemental Documents:
  • pHR-Tre3G-29xGGGGCC-12xMS2.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

29x GGGGCC cannot be confirmed by sequencing due to high GC content. Instability may be observed in the repeat region so it is recommended that multiple DNA preparations be screened.