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PurposeExpresses cAMPr in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 99143 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep2Lox
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Backbone manufacturerMichael Kyba
- Backbone size w/o insert (bp) 2660
- Total vector size (bp) 6042
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Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecAMPr
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Alt namecAMPr, a cp-GFP based sensor for cAMP
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SpeciesM. musculus (mouse)
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Insert Size (bp)2511
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Tag
/ Fusion Protein
- cpGFP
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p2lox-cAMPr was a gift from Justin Blau (Addgene plasmid # 99143 ; http://n2t.net/addgene:99143 ; RRID:Addgene_99143) -
For your References section:
cAMPr: A single-wavelength fluorescent sensor for cyclic AMP. Hackley CR, Mazzoni EO, Blau J. Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. doi: 10.1126/scisignal.aah3738. 10.1126/scisignal.aah3738 PubMed 29511120