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Addgene

U6.3>gRNA.f+e
(Plasmid #99139)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCESAx
  • Total vector size (bp) 3018
  • Modifications to backbone
    Mutated BsaI sites for protospacer cloning
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    gRNA
  • gRNA/shRNA sequence
    Empty
  • Promoter chicken U6.3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer cctataaaaataggcgtatcacg
  • 3′ sequencing primer tggcctgcccggttattattatt
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    U6.3>gRNA.f+e was a gift from Marianne Bronner (Addgene plasmid # 99139 ; http://n2t.net/addgene:99139 ; RRID:Addgene_99139)

  • For your References section:

    Optimization of CRISPR/Cas9 genome editing for loss-of-function in the early chick embryo. Gandhi S, Piacentino ML, Vieceli FM, Bronner ME. Dev Biol. 2017 Dec 1;432(1):86-97. doi: 10.1016/j.ydbio.2017.08.036. 10.1016/j.ydbio.2017.08.036 PubMed 29150011
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