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Addgene

pRRG820-estrella
(Plasmid #99104)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99104 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC53.2
  • Backbone manufacturer
    Phillip Newmark (Addgene plasmid # 26536)
  • Backbone size w/o insert (bp) 3240
  • Vector type
    T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    estrella in situ probe
  • Alt name
    KY024338.1 (Genbank)
  • Alt name
    dd_Smed_v6_1792_0_1
  • Species
    Schmidtea mediterranea
  • Insert Size (bp)
    623

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eam1105I (destroyed during cloning)
  • 3′ cloning site Eam1105I (destroyed during cloning)
  • 5′ sequencing primer T3
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloning primers: F-GAAGTCAATGGAACAGACGA, R-GAATTAGTGAGCGACCAGAA

Note: The estrella insert contains several mismatches compared to KY024338.1. The depositor has confirmed this does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRG820-estrella was a gift from Phillip Newmark (Addgene plasmid # 99104 ; http://n2t.net/addgene:99104 ; RRID:Addgene_99104)

  • For your References section:

    A functional genomics screen in planarians reveals regulators of whole-brain regeneration. Roberts-Galbraith RH, Brubacher JL, Newmark PA. Elife. 2016 Sep 9;5. pii: e17002. doi: 10.7554/eLife.17002. 10.7554/eLife.17002 PubMed 27612384
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