pRRG312-slc1a-5
(Plasmid
#99102)
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PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea slc1a-5 (also known as excitatory amino acid transporter)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 99102 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJC53.2
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Backbone manufacturerPhillip Newmark (Addgene plasmid # 26536)
- Backbone size w/o insert (bp) 3240
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Vector typeT/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameslc1a-5 (also known as excitatory amino acid transporter) in situ probe
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Alt nameKT163445.1 (Genbank)
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Alt namedd_Smed_v6_1106_0_1
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SpeciesSchmidtea mediterranea
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Insert Size (bp)421
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Eam1105I (destroyed during cloning)
- 3′ cloning site Eam1105I (destroyed during cloning)
- 5′ sequencing primer T3
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloning primers: F-gtcaaatgggaaaggaagca R-attaattgtggcgcctatcg
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRRG312-slc1a-5 was a gift from Phillip Newmark (Addgene plasmid # 99102 ; http://n2t.net/addgene:99102 ; RRID:Addgene_99102) -
For your References section:
A functional genomics screen in planarians reveals regulators of whole-brain regeneration. Roberts-Galbraith RH, Brubacher JL, Newmark PA. Elife. 2016 Sep 9;5. pii: e17002. doi: 10.7554/eLife.17002. 10.7554/eLife.17002 PubMed 27612384