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Addgene

pRRG223-smad2/3
(Plasmid #99078)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99078 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC53.2
  • Backbone manufacturer
    Phillip Newmark (Addgene plasmid # 26536)
  • Backbone size w/o insert (bp) 3240
  • Vector type
    T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad2/3 in situ probe
  • Alt name
    KC161225.1 (Genbank)
  • Alt name
    dd_Smed_v6_6079_0_1
  • Species
    Schmidtea mediterranea
  • Insert Size (bp)
    386

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eam1105I (destroyed during cloning)
  • 3′ cloning site Eam1105I (destroyed during cloning)
  • 5′ sequencing primer Sp6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloning primers: F-CATGGAAACAAGCATTGTGG, R-GGTGGGATCTTGCAAACTGT

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRG223-smad2/3 was a gift from Phillip Newmark (Addgene plasmid # 99078 ; http://n2t.net/addgene:99078 ; RRID:Addgene_99078)

  • For your References section:

    Follistatin antagonizes activin signaling and acts with notum to direct planarian head regeneration. Roberts-Galbraith RH, Newmark PA. Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1363-8. doi: 10.1073/pnas.1214053110. Epub 2013 Jan 7. 10.1073/pnas.1214053110 PubMed 23297191
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