pRRG208-ActR1
(Plasmid
#99077)
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PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea ActR1 (activin receptor 1)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 99077 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJC53.2
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Backbone manufacturerPhillip Newmark (Addgene plasmid # 26536)
- Backbone size w/o insert (bp) 3240
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Vector typeT/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameActR1 (activin receptor 1) in situ probe
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Alt nameKC161224.1 (Genbank)
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Alt namedd_Smed_v6_3426_0_1
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SpeciesSchmidtea mediterranea
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Insert Size (bp)484
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Eam1105I (destroyed during cloning)
- 3′ cloning site Eam1105I (destroyed during cloning)
- 5′ sequencing primer Sp6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloning primers: F-GCTGGCTCTTCAAACCAAGA, R-GGTAAACCTGGAATCGCTCA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRRG208-ActR1 was a gift from Phillip Newmark (Addgene plasmid # 99077 ; http://n2t.net/addgene:99077 ; RRID:Addgene_99077) -
For your References section:
Follistatin antagonizes activin signaling and acts with notum to direct planarian head regeneration. Roberts-Galbraith RH, Newmark PA. Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1363-8. doi: 10.1073/pnas.1214053110. Epub 2013 Jan 7. 10.1073/pnas.1214053110 PubMed 23297191