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Addgene

pLV-shIl22ra2-1928
(Plasmid #99033)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99033 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLL3.7
  • Backbone size w/o insert (bp) 7649
  • Total vector size (bp) 7700
  • Vector type
    Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Il22ra2
  • gRNA/shRNA sequence
    GGTGCTCCAACGTATTACT
  • Species
    R. norvegicus (rat)
  • GenBank ID
    NM_001003404
  • Entrez Gene
    Il22ra2 (a.k.a. Crf2-s1)
  • Promoter mouse U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer U6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was generated as part of the NIAAA-funded INIA consortium.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-shIl22ra2-1928 was a gift from Amy Lasek (Addgene plasmid # 99033 ; http://n2t.net/addgene:99033 ; RRID:Addgene_99033)

  • For your References section:

    Reduction of alcohol drinking of alcohol-preferring (P) and high-alcohol drinking (HAD1) rats by targeting phosphodiesterase-4 (PDE4). Franklin KM, Hauser SR, Lasek AW, McClintick J, Ding ZM, McBride WJ, Bell RL. Psychopharmacology (Berl). 2015 Jul;232(13):2251-62. doi: 10.1007/s00213-014-3852-3. Epub 2015 Jan 15. 10.1007/s00213-014-3852-3 [pii] PubMed 25585681
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