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pLV-shScn4b-268
(Plasmid #99031)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99031 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLL3.7
  • Backbone manufacturer
    MIT
  • Backbone size w/o insert (bp) 7649
  • Total vector size (bp) 7700
  • Vector type
    Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Scn4b shRNA
  • gRNA/shRNA sequence
    GACCCTAAGGTGAGAGTGA
  • Species
    M. musculus (mouse)
  • GenBank ID
    NM_001013390.2
  • Entrez Gene
    Scn4b (a.k.a. Gm1471)
  • Promoter mouse U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer U6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was generated as part of the NIAAA-funded INIA consortium.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-shScn4b-268 was a gift from Amy Lasek (Addgene plasmid # 99031 ; http://n2t.net/addgene:99031 ; RRID:Addgene_99031)

  • For your References section:

    The Sodium Channel beta4 Auxiliary Subunit Selectively Controls Long-Term Depression in Core Nucleus Accumbens Medium Spiny Neurons. Ji X, Saha S, Gao G, Lasek AW, Homanics GE, Guildford M, Tapper AR, Martin GE. Front Cell Neurosci. 2017 Feb 13;11:17. doi: 10.3389/fncel.2017.00017. eCollection 2017. 10.3389/fncel.2017.00017 PubMed 28243192

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