pCGT
(Plasmid
#99030)
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Purpose(Empty Backbone) pCG empty vector containing T7 tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 99030 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCG
- Backbone size (bp) 5052
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Modifications to backboneAddition of a T7 tag for N-terminal fusion
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Vector typeMammalian Expression
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Tag
/ Fusion Protein
- T7
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- 5′ sequencing primer CMV-for (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCGT was a gift from Winship Herr (Addgene plasmid # 99030 ; http://n2t.net/addgene:99030 ; RRID:Addgene_99030)