pLV-shMdk-334
(Plasmid
#99025)
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PurposeExpresses shRNA targeting mouse Mdk (midkine)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99025 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLL3.7
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Backbone manufacturerMIT
- Backbone size w/o insert (bp) 7649
- Total vector size (bp) 7700
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Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMdk shRNA
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gRNA/shRNA sequenceGAGCCGACTGCAAATACAA
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SpeciesM. musculus (mouse)
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GenBank IDNM_010784
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Entrez GeneMdk (a.k.a. MK, Mek)
- Promoter mouse U6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer U6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was generated as part of the NIAAA-funded INIA consortium.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-shMdk-334 was a gift from Amy Lasek (Addgene plasmid # 99025 ; http://n2t.net/addgene:99025 ; RRID:Addgene_99025) -
For your References section:
Midkine in the mouse ventral tegmental area limits ethanol intake and Ccl2 gene expression. Chen H, He D, Lasek AW. Genes Brain Behav. 2017 Apr 11. doi: 10.1111/gbb.12384. 10.1111/gbb.12384 PubMed 28398003
