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PurposeMammalian expression of NS1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 99022 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCX
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNS1
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Insert Size (bp)654
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCX-V5-H7N9-NS1 was a gift from Honglin Chen (Addgene plasmid # 99022 ; http://n2t.net/addgene:99022 ; RRID:Addgene_99022) -
For your References section:
An NS-segment exonic splicing enhancer regulates influenza A virus replication in mammalian cells. Huang X, Zheng M, Wang P, Mok BW, Liu S, Lau SY, Chen P, Liu YC, Liu H, Chen Y, Song W, Yuen KY, Chen H. Nat Commun. 2017 Mar 21;8:14751. doi: 10.1038/ncomms14751. 10.1038/ncomms14751 PubMed 28323816