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Addgene

pcDNA-FLAG-SF2
(Plasmid #99021)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99021 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SF2
  • Alt name
    ASF
  • Alt name
    SRSF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    744
  • Entrez Gene
    SRSF1 (a.k.a. ASF, NEDFBA, SF2, SF2p33, SFRS1, SRp30a)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-FLAG-SF2 was a gift from Honglin Chen (Addgene plasmid # 99021 ; http://n2t.net/addgene:99021 ; RRID:Addgene_99021)

  • For your References section:

    An NS-segment exonic splicing enhancer regulates influenza A virus replication in mammalian cells. Huang X, Zheng M, Wang P, Mok BW, Liu S, Lau SY, Chen P, Liu YC, Liu H, Chen Y, Song W, Yuen KY, Chen H. Nat Commun. 2017 Mar 21;8:14751. doi: 10.1038/ncomms14751. 10.1038/ncomms14751 PubMed 28323816
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