YFP Rab4DN
(Plasmid #99019)

• Purpose: Localization and visualization of the dominant negative form of Rab4
• Depositing Lab: Narasimhan Gautam
• Publication: O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(18)30422-2. doi: 10.1016/j.devcel.2018.05.029.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6790
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rab4 DN
• Species: D. melanogaster (fly), Synthetic
• Insert Size (bp): 789
• Mutation: S22N Dominant negative
• GenBank ID: NM_166240.3
• Entrez Gene: Rab4 (a.k.a. Dmel_CG4921, CG 4921, CG4921, DRAB4, DmRab4, Dmel\CG4921, Q7KY04, RAB4c, rab4)
• Promoter: CMV
• Tag / Fusion Protein:
  • YFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Matthew Scott Addgene #37691

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

YFP Rab4DN from Addgene 37691 was transferred to pcDNA3.1