mCherry-KRasCT
(Plasmid #99017)

• Purpose: Membrane marker
• Depositing Lab: Narasimhan Gautam
• Publication: O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(18)30422-2. doi: 10.1016/j.devcel.2018.05.029.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6217
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry KRas CT
• Species: Synthetic
• Insert Size (bp): 789
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The mCherry KRasCT fragment was made as a gBlock with HindIII and XbaI, cut and cloned into pcDNA3.1

Please note that the sequence lacks the last two amino acids of mCherry and has a modified KRasCT sequence. The plasmid functions as described in the publication.