pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1
(Plasmid
#98972)
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PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 98972 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepX460
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Backbone manufacturerFeng Zhang
- Total vector size (bp) 6815
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLMNA sgRNAs pair 1 and Cas9(D10A)
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SpeciesH. sapiens (human)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1 was a gift from Jan Karlseder (Addgene plasmid # 98972 ; http://n2t.net/addgene:98972 ; RRID:Addgene_98972) -
For your References section:
Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN. Arnoult N, Correia A, Ma J, Merlo A, Garcia-Gomez S, Maric M, Tognetti M, Benner CW, Boulton SJ, Saghatelian A, Karlseder J. Nature. 2017 Sep 20;549(7673):548-552. doi: 10.1038/nature24023. 10.1038/nature24023 PubMed 28959974
Map uploaded by the depositor.
