pCEP4-myc-CCR5-VC
(Plasmid #98966)

• Purpose: myc-tagged human CCR5 fused to C-terminus of split Venus
• Depositing Lab: Erik Procko
• Publication: Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CCR5
• Alt name: CD195
• Species: H. sapiens (human)
• Insert Size (bp): 1410
• Mutation: Full-length, wildtype
• GenBank ID: NM_000579
• Entrez Gene: CCR5 (a.k.a. CC-CKR-5, CCCKR5, CCR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Signal/leader sequence from Influenza A virus HA protein (N terminal on insert)
  • c-myc epitope tag (N terminal on insert)
  • VC: Venus (I152L) C-terminal residues 155-238 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV_fwd
• 3′ sequencing primer: EBV_rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry