pCEP4-FLAG-GRM3-VN
(Plasmid #98965)

• Purpose: FLAG-tagged human mGluR3 fused to N-terminus of split Venus
• Depositing Lab: Erik Procko
• Publication: Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10393
• Total vector size (bp): 13525
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GRM3
• Alt name: mGluR3
• Species: H. sapiens (human)
• Insert Size (bp): 3132
• Mutation: Full-length, wildtype
• GenBank ID: NM_000840.2
• Entrez Gene: GRM3 (a.k.a. GLUR3, GPRC1C, MGLUR3, mGlu3)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Signal/leader sequence from HLA class I A-2 alpha chain (N terminal on insert)
  • FLAG (dykddddk) epitope tag (N terminal on insert)
  • VN: Venus (I152L) N-terminal residues 1-154 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV_fwd
• 3′ sequencing primer: EBV_rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry