pCEP4-gp120(BaL)-CD4
(Plasmid #98957)

• Purpose: Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate
• Depositing Lab: Erik Procko
• Publication: Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10393
• Total vector size (bp): 12601
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HIV-1 (BaL) gp120
• Alt name: Env
• Species: Human immunodeficiency virus 1
• Insert Size (bp): 2208
• Mutation: Codon-optimized synthetic gene
• GenBank ID: AAA44191.1
• Promoter: CMV
• Tags / Fusion Proteins:
  • CD5 leader sequence (N terminal on insert)
  • CD4 (domains D1-D2) (C terminal on insert)
  • avi tag for enzymatic biotinylation (C terminal on insert)
  • FLAG epitope tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV_fwd
• 3′ sequencing primer: EBV_rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry