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pCEP4-CCR5(R5gp120-Hi)
(Plasmid #98954)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98954 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CCR5
  • Alt name
    CD195
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1161
  • Mutation
    R5gp120-Hi variant: P8L, I9E, I12M, S17E, P19D, Q21E, V51N, L80A, S270E
  • GenBank ID
    NM_000579
  • Entrez Gene
    CCR5 (a.k.a. CC-CKR-5, CCCKR5, CCR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Signal/leader sequence from Influenza A virus HA protein (N terminal on insert)
    • c-myc epitope tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV_fwd
  • 3′ sequencing primer EBV_rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-CCR5(R5gp120-Hi) was a gift from Erik Procko (Addgene plasmid # 98954 ; http://n2t.net/addgene:98954 ; RRID:Addgene_98954)

  • For your References section:

    Mapping Interaction Sites on Human Chemokine Receptors by Deep Mutational Scanning. Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E. J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343. 10.4049/jimmunol.1800343 PubMed 29678950
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