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Addgene

pZa-tetO-csgAWY-CmR
(Plasmid #98939)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98939 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZa-tetO-CmR
  • Backbone size w/o insert (bp) 2274
  • Total vector size (bp) 2763
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    csgAWY
  • Insert Size (bp)
    489
  • GenBank ID
    NC_000913.3
  • Promoter tetO
  • Tag / Fusion Protein
    • YYRKYKEWDDY (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer TGGTGAAAGTTGGAACCTCTT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZa-tetO-csgAWY-CmR was a gift from Urartu Seker (Addgene plasmid # 98939 ; http://n2t.net/addgene:98939 ; RRID:Addgene_98939)

  • For your References section:

    Genetically encoded conductive protein nanofibers secreted by engineered cells. Kalyoncu, Ebuzer, Recep Ahan, Tolga T. Olmez, and Urartu Ozgur Safak Seker. RSC Advances, Issue 52, 2017 10.1039/C7RA06289C
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