pIW601-KmCRISPR
(Plasmid #98907)

• Purpose: K. marxianus CRISPR Plasmid for sgRNA cloning
• Depositing Lab: Ian Wheeldon
• Publication: Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164. doi: 10.1186/s13068-017-0854-5. eCollection 2017.

Backbone

• Vector backbone: pJSK316-GPD
• Backbone size w/o insert (bp): 5558
• Total vector size (bp): 11093
• Vector type: Yeast Expression, CRISPR, Synthetic Biology
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Codon optimized Cas9
• Species: Synthetic; K. marxianus
• Insert Size (bp): 4140
• Tag / Fusion Protein:
  • SV40 (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: sgRNA expression cassette
• Species: Synthetic
• Insert Size (bp): 693

Resource Information

• Supplemental Documents:
  • 2016_04_29 piW601 pJSK316-CRISPR-Tef1KmCas-KmRPR1p-GlytRNA CRISPR BACKBONE.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Easy cloning vector: Cut backbone with PspXI for Gibson Assembly with annealed primers of 20bp (up- and downstream) to insert 20bp target sequence .