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Addgene

pIW447-CRISPR XYL2
(Plasmid #98906)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98906 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJSK316-GPD
  • Backbone size w/o insert (bp) 5558
  • Total vector size (bp) 11113
  • Vector type
    Yeast Expression, CRISPR, Synthetic Biology
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Codon optimized Cas9
  • Species
    Synthetic; K. marxianus
  • Insert Size (bp)
    4140
  • Tag / Fusion Protein
    • SV40 (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    sgRNA expression cassette
  • Species
    Synthetic
  • Insert Size (bp)
    713

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIW447-CRISPR XYL2 was a gift from Ian Wheeldon (Addgene plasmid # 98906 ; http://n2t.net/addgene:98906 ; RRID:Addgene_98906)

  • For your References section:

    CRISPR-Cas9-enabled genetic disruptions for understanding ethanol and ethyl acetate biosynthesis in Kluyveromyces marxianus. Lobs AK, Engel R, Schwartz C, Flores A, Wheeldon I. Biotechnol Biofuels. 2017 Jun 24;10:164. doi: 10.1186/s13068-017-0854-5. eCollection 2017. 10.1186/s13068-017-0854-5 PubMed 28652865
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