pET42a-lambdaN+-L+-GSH
(Plasmid #98894)

• Purpose: Expresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo method
• Depositing Lab: Pascale Legault
• Publication: Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. doi: 10.1093/nar/gkq1084. Epub 2010 Nov 11.

Backbone

• Vector backbone: pET42a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5930
• Total vector size (bp): 6065
• Modifications to backbone: stop codon inserted after the GST tag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: For expression, depositing laboratory recommends strain BL21 (DE3) with 4 hours IPTG induction
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: lambdaN+-L+
• Species: Synthetic
• Insert Size (bp): 129
• Promoter: T7 promoter
• Tag / Fusion Protein:
  • GST tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: NdeI (destroyed during cloning)
• 5′ sequencing primer: T7 promoter, forward primer

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry