pB7WG_ASX
(Plasmid #98891)

• Purpose: (Empty Backbone) Plant expression vector for multigene cloning
• Depositing Lab: Kristina Gruden
• Publication: Lukan et al PLoS One. 2018 Jan 4;13(1):e0190526. doi: 10.1371/journal.pone.0190526. eCollection 2018.

Backbone

• Vector backbone: pB7WG
• Backbone manufacturer: VIB
• Backbone size (bp): 9915
• Modifications to backbone: Release of T35S–AttR2–ccdB–AttR1 cassette from the vector backbone. Introduction of I-SceI–A0–HindIII–ccdB–HindIII–B0–I-SceI cassette, to add A0 and B0 AssemblX compatible homology regions with flanking restriction enzymes recognition sites and the ccdB gene. pB7WG_ASX is compatible with AssemblX cloning system.
• Vector type: Plant Expression, Synthetic Biology
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: /
• Copy number: High Copy

Cloning Information

• 5′ sequencing primer: 5'-TAGGGATAACAGGGTAATATC-3'

Resource Information

• Supplemental Documents:
  • pB7WG_ASX.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry