pCAMBIA_ASX
(Plasmid #98888)

• Purpose: (Empty Backbone) Plant expression vector for multigene cloning
• Depositing Lab: Kristina Gruden
• Publication: Lukan et al PLoS One. 2018 Jan 4;13(1):e0190526. doi: 10.1371/journal.pone.0190526. eCollection 2018.

Backbone

• Vector backbone: pCAMBIA1300
• Backbone manufacturer: Cambia
• Backbone size (bp): 8959
• Modifications to backbone: Introduction of I-SceI–A0–HindIII–ccdB–HindIII–B0–I-SceI cassette to add AssemblX compatible homology regions A0 and B0 with flanking restriction enzyme recognition sites and ccdB gene. pCAMBIA_ASX is compatible with AssemblX cloning strategy.
• Vector type: Plant Expression, Synthetic Biology
• Promoter: CaMV35S (enhanced)
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: /
• Copy number: High Copy

Cloning Information

• 5′ sequencing primer: M13 Reverse
• 3′ sequencing primer: M13 Forward

Resource Information

• Supplemental Documents:
  • pCAMBIA_ASX.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry