pN-iRS3GG
(Plasmid #98858)

• Purpose: Expresses pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).
• Depositing Lab: Lydia Contreras
• Publication: Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):5523-5538. doi: 10.1093/nar/gkx115.

Backbone

• Vector backbone: pCML 521
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Escherichia coli str. K-12 substr. MG1655 is recommended by the depositor for expression
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PheS cassette to be selectively replaced by unique asRNA probes
• Insert Size (bp): 1087
• Promoter: pBAD

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmbI (not destroyed)
• 3′ cloning site: BsmbI (not destroyed)
• 5′ sequencing primer: CCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGC

Resource Information

• Supplemental Documents:
  • n-iRS3GG.gb
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that a 6bp deletion was found during Addgene's quality control which eliminates the XhoI site (CTCGAG). The depositing lab noted there are additional sites present for cloning, and disruption of XhoI does NOT affect plasmid function.