Dnase1L3 R206C pIRES-DsRed
(Plasmid #98847)

• Purpose: Expresses Human Dnase1L3 with activity-reducing HUVS mutation R206C in mammalian cells along with DsRed
• Depositing Lab: Peter Keyel
• Publication: Shi et al Front Immunol. 2017 May 8;8:522. doi: 10.3389/fimmu.2017.00522. eCollection 2017.

Backbone

• Vector backbone: pIRES2-DsRed2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 6200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Dnase1L3
• Alt name: Dnase gamma
• Species: H. sapiens (human)
• Insert Size (bp): 914
• Mutation: R206C which greatly reduces nuclease activity
• GenBank ID: DQ896097.2
• Entrez Gene: DNASE1L3 (a.k.a. DHP2, DNAS1L3, LSD, SLEB16)
• Promoter: CMV
• Tag / Fusion Protein:
  • IRES DsRed

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV forward (Genewiz Universal)
• 3′ sequencing primer: IRES- (custom)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dnase1L3 cloned by PCR initially from Open Biosystems

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry