pLK89 - Edit-directing plasmid cloning insert
(Plasmid
#98846)
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PurposeContains the insert used for step 2 of edit-directed plasmid cloning
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98846 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS426
- Total vector size (bp) 7298
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Vector typeYeast Expression, CRISPR
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsAlso encodes resistance to Kanamycin
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKanR
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Insert Size (bp)795
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MutationChanged sequence to remove a MluI cut site
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLK89 - Edit-directing plasmid cloning insert was a gift from Leonid Kruglyak (Addgene plasmid # 98846 ; http://n2t.net/addgene:98846 ; RRID:Addgene_98846)