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pLK88 - Edit-directing plasmid base
(Plasmid #98814)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98814 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS426
  • Total vector size (bp) 6274
  • Modifications to backbone
    gRNA
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAN1.y gRNA
  • gRNA/shRNA sequence
    GATACGTTCTCTATGGAGGA

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Derived from p426-SNR52p-gRNA.CAN1.Y-SUP4t, provided by George Church. Contains modifications to the gRNA promoter and structural region that incorporate a BstEII and SphI site for cloning purposes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLK88 - Edit-directing plasmid base was a gift from Leonid Kruglyak (Addgene plasmid # 98814 ; http://n2t.net/addgene:98814 ; RRID:Addgene_98814)
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