MSP972
(Plasmid
#98806)
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PurposeExpression Vector
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Depositing Lab
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 98806 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBR322
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Backbone manufacturerATCC
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepET28 MBP-ENLYFQS-TEV T17S, N68D, E107D, D127A, F132L, S135F, F162S, K229E
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SpeciesSynthetic
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MutationT17S, N68D, E107D, D127A, F132L, S135F, F162S, K229E
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSP972 was a gift from David Liu (Addgene plasmid # 98806 ; http://n2t.net/addgene:98806 ; RRID:Addgene_98806) -
For your References section:
Phage-assisted continuous evolution of proteases with altered substrate specificity. Packer MS, Rees HA, Liu DR. Nat Commun. 2017 Oct 16;8(1):956. doi: 10.1038/s41467-017-01055-9. 10.1038/s41467-017-01055-9 [pii] PubMed 29038472