MSP832
(Plasmid
#98792)
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PurposeAccessory Plasmid for PACE
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 98792 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSC101
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Backbone manufacturerATCC
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameproB-lysozyme-ggs-HPLVGHM-ggs-T7RNAP(Q649S)//T7pro-gIII-lux
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SpeciesSynthetic
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MutationNone
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSP832 was a gift from David Liu (Addgene plasmid # 98792 ; http://n2t.net/addgene:98792 ; RRID:Addgene_98792) -
For your References section:
Phage-assisted continuous evolution of proteases with altered substrate specificity. Packer MS, Rees HA, Liu DR. Nat Commun. 2017 Oct 16;8(1):956. doi: 10.1038/s41467-017-01055-9. 10.1038/s41467-017-01055-9 [pii] PubMed 29038472