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Addgene

pSimple-miR302/367
(Plasmid #98748)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98748 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSimpleII
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miR302/367 (miRNA cluster)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1083
  • Entrez Gene
    MIR302A (a.k.a. MIRN302, MIRN302A, hsa-mir-302, mir-302a)
  • Entrez Gene
    MIR367 (a.k.a. MIRN367, hsa-mir-367)
  • Promoter EF1alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use in addition to episomal reprogramming vectors

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSimple-miR302/367 was a gift from Sara Howden & James Thomson (Addgene plasmid # 98748 ; http://n2t.net/addgene:98748 ; RRID:Addgene_98748)

  • For your References section:

    Simultaneous Reprogramming and Gene Correction of Patient Fibroblasts. Howden SE, Maufort JP, Duffin BM, Elefanty AG, Stanley EG, Thomson JA. Stem Cell Reports. 2015 Dec 8;5(6):1109-18. doi: 10.1016/j.stemcr.2015.10.009. Epub 2015 Nov 12. 10.1016/j.stemcr.2015.10.009 PubMed 26584543
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