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pRL_GI_PCB28
(Plasmid #98745)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98745 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pL0C_4-R
  • Backbone size w/o insert (bp) 5864
  • Total vector size (bp) 9733
  • Modifications to backbone
    Insertion of a TRP marker, Insertion of a genome integration cassette for the his1-Δ200 locus
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    TRP1, URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    heme oxygenase 1
  • Alt name
    HY1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    849
  • GenBank ID
    AB021858
  • Entrez Gene
    TED4 (a.k.a. AT2G26670, ARABIDOPSIS THALIANA HEME OXYGENASE 1, ATHO1, F18A8.4, F18A8_4, GENOMES UNCOUPLED 2, GUN2, HEME OXYGENASE, HEME OXYGENASE 1, HEME OXYGENASE 6, HO1, HY1, HY6, PLASTID HEME OXYGENASE, REVERSAL OF THE DET PHENOTYPE 4)
  • Promoter PGK1

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGTAAAGGATGGGGAAAGAGA
  • 3′ sequencing primer GTTTAGTAGAACCTCGTG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Phycocyanobilin:ferredoxin oxidoreductase
  • Alt name
    PcyA
  • Species
    Synechocystis sp. PCC 6803
  • Insert Size (bp)
    747
  • Mutation
    Codon optimized for Saccharomyces cerevisiae
  • Promoter ADH1m

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCCTTTCTTCCTTGTTTC
  • 3′ sequencing primer GGTGTGGTCAATAAGAGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRL_GI_PCB28 was a gift from Bernd Müller-Röber (Addgene plasmid # 98745 ; http://n2t.net/addgene:98745 ; RRID:Addgene_98745)

  • For your References section:

    PhiReX: a programmable and red light-regulated protein expression switch for yeast. Hochrein L, Machens F, Messerschmidt K, Mueller-Roeber B. Nucleic Acids Res. 2017 Sep 6;45(15):9193-9205. doi: 10.1093/nar/gkx610. 10.1093/nar/gkx610 PubMed 28911120
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