GLT-1b delta 4
(Plasmid
#98707)
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Purposemammalian expression of Glt1b delta 4 mutant
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98707 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGlt1b
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SpeciesR. norvegicus (rat)
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Mutationmissing last 4 amino acids at the C-terminus. I521V and N538Y (please see depositor comments section below)
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Entrez GeneSlc1a2 (a.k.a. Eaat2, Glt, Glt-1)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that mutations I521V and N538Y in Glt1b (reference AF451299) were found during Addgene's quality control. The depositor noted that these mutations do NOT affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GLT-1b delta 4 was a gift from Paul Rosenberg (Addgene plasmid # 98707 ; http://n2t.net/addgene:98707 ; RRID:Addgene_98707)