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Addgene

pAAV-IV-GFPL10
(Plasmid #98704)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98704 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
  • Backbone manufacturer
    Deisseroth Lab
  • Backbone size w/o insert (bp) 5524
  • Total vector size (bp) 7158
  • Modifications to backbone
    3' Lox sites moved
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFPL10
  • Alt name
    EGFPL10a
  • Species
    M. musculus (mouse); A. victoria (jellyfish)
  • Insert Size (bp)
    1634
  • Mutation
    L10: 2609 C->T to remove EcoRI site
  • Promoter EF1a
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EF1a-Fwd
  • 3′ sequencing primer WPRE-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-IV-GFPL10 was a gift from Jeffrey Friedman & Alexander Nectow (Addgene plasmid # 98704 ; http://n2t.net/addgene:98704 ; RRID:Addgene_98704)

  • For your References section:

    Identification of a Brainstem Circuit Controlling Feeding. Nectow AR, Schneeberger M, Zhang H, Field BC, Renier N, Azevedo E, Patel B, Liang Y, Mitra S, Tessier-Lavigne M, Han MH, Friedman JM. Cell. 2017 Jul 27;170(3):429-442.e11. doi: 10.1016/j.cell.2017.06.045. 10.1016/j.cell.2017.06.045 PubMed 28753423
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