pAAV-IV-GFPL10
(Plasmid #98704)

• Purpose: Cre-dependently express GFPL10 fusion using Introvert Switch
• Depositing Labs: Jeffrey Friedman, Alexander Nectow
• Publication: Nectow et al Cell. 2017 Jul 27;170(3):429-442.e11. doi: 10.1016/j.cell.2017.06.045.

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Backbone

• Vector backbone: pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
• Backbone manufacturer: Deisseroth Lab
• Backbone size w/o insert (bp): 5524
• Total vector size (bp): 7158
• Modifications to backbone: 3' Lox sites moved
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFPL10
• Alt name: EGFPL10a
• Species: M. musculus (mouse); A. victoria (jellyfish)
• Insert Size (bp): 1634
• Mutation: L10: 2609 C->T to remove EcoRI site
• Promoter: EF1a
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EF1a-Fwd
• 3′ sequencing primer: WPRE-Rev

Resource Information

• Supplemental Documents:
  • pAAV-IV-GFPL10.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry