pCS2+ gfp-zASC
(Plasmid #98703)

• Purpose: GFP tagged zebrafish ASC in the pCS2+ vector for in vitro transcription
• Depositing Lab: Anna Huttenlocher
• Publication: Vincent et al Cell Microbiol. 2016 Apr;18(4):591-604. doi: 10.1111/cmi.12536. Epub 2015 Nov 4.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Total vector size (bp): 5430
• Modifications to backbone: Multiple cloning site was shortened during insertion of the construct
• Vector type: Mammalian Expression ; Zebrafish/Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Apoptosis associated speck like protein containing CARD
• Alt name: ASC
• Alt name: PYCARD
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1357
• GenBank ID: NC_007127.6
• Entrez Gene: pycard (a.k.a. ASC, asc1, zAsc, zgc:109874)
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: This plasmid has an I78N mutation compared to the depositor's sequence and reference sequence. The functional impact of this mutation has not been verified.