sox10:PDGFRA mutant
(Plasmid
#98697)
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PurposeI-SceI meganuclease-mediated human PDGFRA mutant transgenesis in zebrafish
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98697 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST
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Backbone manufacturerInvitrogen
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Vector typeZebrafish Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePDGFRA
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SpeciesH. sapiens (human)
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Mutationexon 8 and 9 deletion
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Entrez GenePDGFRA (a.k.a. CD140A, PDGFR-2, PDGFR2)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
sox10:PDGFRA mutant was a gift from Thomas Look (Addgene plasmid # 98697 ; http://n2t.net/addgene:98697 ; RRID:Addgene_98697) -
For your References section:
Overexpression of PDGFRA cooperates with loss of NF1 and p53 to accelerate the molecular pathogenesis of malignant peripheral nerve sheath tumors. Ki DH, He S, Rodig S, Look AT. Oncogene. 2017 Feb 23;36(8):1058-1068. doi: 10.1038/onc.2016.269. Epub 2016 Aug 1. 10.1038/onc.2016.269 PubMed 27477693