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pLD-puro-Cc-PRDX5K83R-VA
(Plasmid #98690)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98690 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLD-puro-CcVA (Addgene plasmid #24588)
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRDX5
  • Alt name
    Peroxiredoxin5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    642
  • Mutation
    K83R
  • GenBank ID
    NM_012094.4
  • Entrez Gene
    PRDX5 (a.k.a. ACR1, AOEB166, B166, HEL-S-55, PLP, PMP20, PRDX6, PRXV, SBBI10, prx-V)
  • Promoter CMV
  • Tag / Fusion Protein
    • VA tag (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hPGK_R [5'-ccttggaaaaggcgcaacc-3']
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Mammalian Gene Collection team, ORFeome available from Harvard Plasmid ID, clone HsCD00366825

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The C terminus VA tag (C terminal on the tagged protein) consists of the following features: Beacon tag [ELFNLLGENQPPVVIK] - 2XStrepII - 6XHis - 2XTEV - 3XFlag

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLD-puro-Cc-PRDX5K83R-VA was a gift from Mohan Babu (Addgene plasmid # 98690 ; http://n2t.net/addgene:98690 ; RRID:Addgene_98690)

  • For your References section:

    A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-kappaB Signaling. Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson J, Babu M. Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 10.1016/j.cels.2017.10.010. 10.1016/j.cels.2017.10.010 PubMed 29128334
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