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Addgene

TDP-43_CTD_Q331K
(Plasmid #98673)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98673 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ411
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TDP-43
  • Species
    H. sapiens (human)
  • Mutation
    Q331K
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TDP-43_CTD_Q331K was a gift from Nicolas Fawzi (Addgene plasmid # 98673 ; http://n2t.net/addgene:98673 ; RRID:Addgene_98673)

  • For your References section:

    ALS Mutations Disrupt Phase Separation Mediated by alpha-Helical Structure in the TDP-43 Low-Complexity C-Terminal Domain. Conicella AE, Zerze GH, Mittal J, Fawzi NL. Structure. 2016 Sep 6;24(9):1537-49. doi: 10.1016/j.str.2016.07.007. Epub 2016 Aug 18. 10.1016/j.str.2016.07.007 PubMed 27545621
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