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PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98669 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJ411
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTDP-43
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SpeciesH. sapiens (human)
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Entrez GeneTARDBP (a.k.a. ALS10, TDP-43)
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Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TDP-43_CTD was a gift from Nicolas Fawzi (Addgene plasmid # 98669 ; http://n2t.net/addgene:98669 ; RRID:Addgene_98669) -
For your References section:
ALS Mutations Disrupt Phase Separation Mediated by alpha-Helical Structure in the TDP-43 Low-Complexity C-Terminal Domain. Conicella AE, Zerze GH, Mittal J, Fawzi NL. Structure. 2016 Sep 6;24(9):1537-49. doi: 10.1016/j.str.2016.07.007. Epub 2016 Aug 18. 10.1016/j.str.2016.07.007 PubMed 27545621