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MBP-hnRNPA2_FL_D290V
(Plasmid #98663)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98663 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTHMT
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hnRNPA2
  • Species
    H. sapiens (human)
  • Mutation
    D290V
  • Entrez Gene
    HNRNPA2B1 (a.k.a. HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, IBMPFD2, OPMD2, RNPA2, SNRPB1)
  • Tag / Fusion Protein
    • 6xHis-MBP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MBP-hnRNPA2_FL_D290V was a gift from Nicolas Fawzi (Addgene plasmid # 98663 ; http://n2t.net/addgene:98663 ; RRID:Addgene_98663)

  • For your References section:

    Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, Interactions, and Phase Separation Altered by Mutation and Arginine Methylation. Ryan VH, Dignon GL, Zerze GH, Chabata CV, Silva R, Conicella AE, Amaya J, Burke KA, Mittal J, Fawzi NL. Mol Cell. 2018 Jan 17. pii: S1097-2765(17)30979-6. doi: 10.1016/j.molcel.2017.12.022. 10.1016/j.molcel.2017.12.022 PubMed 29358076
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