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pCR8/GW/TOPO-ELK3
(Plasmid #98622)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98622 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR8/GW/TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2817
  • Vector type
    TOPO TA cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ELK3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1230
  • Mutation
    E167K
  • Entrez Gene
    Elk3 (a.k.a. D430049E23Rik, Erp, Etrp, Net, Sap-2)

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert contains Kozak sequence and no stop codon.

Addgene's sequencing found an E167K mutation in ELK3. This mutation is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR8/GW/TOPO-ELK3 was a gift from Richard Harvey (Addgene plasmid # 98622 ; http://n2t.net/addgene:98622 ; RRID:Addgene_98622)

  • For your References section:

    NKX2-5 mutations causative for congenital heart disease retain functionality and are directed to hundreds of targets. Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA, Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, Harvey RP. Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942. 10.7554/eLife.06942 PubMed 26146939
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