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pENTR-2B-Nkx2-5 Y191C
(Plasmid #98613)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98613 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR 2B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2718
  • Vector type
    Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NKX2-5 Y191C
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    957
  • Mutation
    Point mutation Y191C
  • Entrez Gene
    Nkx2-5 (a.k.a. Csx, Nkx-2.5, Nkx2.5, tinman)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The mouse NKX2-5Y191C mutant was generated by site-directed mutagenesis of the Nkx2-5 cDNA using the following primer and its reverse complement: TCCAGAACCGTCGCTGCAAGTGCAAGCGACAG. Insert contains Kozak sequence and no stop codon

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-2B-Nkx2-5 Y191C was a gift from Richard Harvey (Addgene plasmid # 98613 ; http://n2t.net/addgene:98613 ; RRID:Addgene_98613)

  • For your References section:

    NKX2-5 mutations causative for congenital heart disease retain functionality and are directed to hundreds of targets. Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA, Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, Harvey RP. Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942. 10.7554/eLife.06942 PubMed 26146939
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