pLgw EcoDam-V5-ELK1Y158A
(Plasmid
#98608)
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PurposeMammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 98608 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLgw EcoDam-V5-RFC1
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Backbone manufacturerPMID 9733864
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Vector typeMammalian Expression, Lentiviral ; DamID
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameELK1Y158A
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SpeciesM. musculus (mouse)
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Entrez GeneElk1 (a.k.a. Elk-1)
- Promoter Heat Shock Minimal Promoter
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Tag
/ Fusion Protein
- Dam (DNA adenine methyltransferase) and V5 (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLgw EcoDam-V5-ELK1Y158A was a gift from Richard Harvey (Addgene plasmid # 98608 ; http://n2t.net/addgene:98608 ; RRID:Addgene_98608)