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pLgw EcoDam-V5-NKX2-5Y191C
(Plasmid #98598)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98598 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLgw EcoDam-V5-RFC1
  • Backbone manufacturer
    PMID 9733858
  • Vector type
    Mammalian Expression, Lentiviral ; DamID

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NKX2-5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    957
  • Mutation
    Y191C point mutant
  • Entrez Gene
    Nkx2-5 (a.k.a. Csx, Nkx-2.5, Nkx2.5, tinman)
  • Promoter Heat Shock Minimal Promoter
  • Tag / Fusion Protein
    • Dam (DNA adenine methyltransferase) and V5 (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLgw EcoDam-V5-NKX2-5Y191C was a gift from Richard Harvey (Addgene plasmid # 98598 ; http://n2t.net/addgene:98598 ; RRID:Addgene_98598)

  • For your References section:

    NKX2-5 mutations causative for congenital heart disease retain functionality and are directed to hundreds of targets. Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA, Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, Harvey RP. Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942. 10.7554/eLife.06942 PubMed 26146939
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