p-OiRS3GG
(Plasmid #98589)

• Purpose: Expresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).
• Depositing Lab: Lydia Contreras
• Publication: Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):5523-5538. doi: 10.1093/nar/gkx115.

Backbone

• Vector backbone: pCML193
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Escherichia coli str. K-12 substr. MG1655 is recommended by the depositor for expression
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: group I intron
• Species: Tetrahymena
• Insert Size (bp): 393
• Promoter: pBAD

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGC

Gene/Insert 2

• Gene/Insert name: PheS cassette to be selectively replaced by unique asRNA probes
• Insert Size (bp): 1087
• Promoter: pLtetO

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmbI (not destroyed)
• 3′ cloning site: BsmbI (not destroyed)
• 5′ sequencing primer: CGAGTCCCTATCAGTGATAGAGATTGACATCCC

Resource Information

• Supplemental Documents:
  • GG iRS3_SS annotation.gb
• A portion of this plasmid was derived from a plasmid made by: Dr. Russel, UT Austin (group I intron)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry