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MAP1LC3A-V1
(Plasmid #98569)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98569 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2-AcGFP
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 5966
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAP1LC3A
  • Alt name
    LC3A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    366
  • GenBank ID
    NM_032514
  • Entrez Gene
    MAP1LC3A (a.k.a. ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer AAAAAGAATTCATGCCCTCAGACCGGCC
  • 3′ sequencing primer AAAAAGGATCCTCAGAAGCCGAAGGTTTCC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAP1LC3A-V1 was a gift from Shahenda El-Naggar (Addgene plasmid # 98569 ; http://n2t.net/addgene:98569 ; RRID:Addgene_98569)

  • For your References section:

    LC3A Silencing Hinders Aggresome Vimentin Cage Clearance in Primary Choroid Plexus Carcinoma. Nassar M, Samaha H, Ghabriel M, Yehia M, Taha H, Salem S, Shaaban K, Omar M, Ahmed N, El-Naggar S. Sci Rep. 2017 Aug 14;7(1):8022. doi: 10.1038/s41598-017-07403-5. 10.1038/s41598-017-07403-5 [pii] PubMed 28808307
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